Step 6: prepare and apply the PGM- specific stain to the gel
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obtain a glass test tube and put a coating of aluminum foil around the tube
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add 1.0 ml tris-HCL buffer, 1.5 ml NAD, 5 drops McCl2, 5 drops Glucose-1-phosphate, 5 drops of MTT to the test tube.
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cover tube with parafilm and mix for a 2 minutes
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wait until your gel is almost ready to be taken out of the chamber before continuing to next step
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2 minutes before get is ready to be removed, add 5 drops of PMS and 20 micro liters of G6PDH to test tube
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IMMEDIATELY pour solution over the center of gel so that stain flows over entire gel
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pipette 2 ml of molten agar into test tube with stain solution
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place dull surface of gel up into one of staining trays
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locate plastic staining trays adjacent to the beaker of molten agar
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continue mixing until gel is removed
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DO NOT MOVE STAINING TRAY
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place dark cover "ice bucket" over staining tray for 10 minutes
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bilogy
