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Science & NatureMilk Lab
By magull
Category: Science & Nature. Viewed 1183 times. Created October 2013.     Disclaimer.   
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Add about 50 mL of milk to a beaker. Record mass.
Add about 1 mL of 8 M Acetic Acid to the milk and stir while heating on hotplate at medium setting.
Once it boils remove and let cool for about 10 minutes.
Pour the beaker through a cheese cloth and into a 250 mL beaker. Squeeze the cheese cloth with the fat in it to get excess milk out.
Place precipitate from cheese cloth into a 150 mL beaker.

Add about 7mL of 1 M NaOH to the filtrate and test with litmus paper to ensure it is basic.
Place casein on a watch glass.
Separate the casein from the fat by using your buchner funnel. and wash with 5 mL of acetone.
Break the precipitate apart and then mix while on hot plate at low setting until it boils. take off and let cool for 2 minutes. Repeat 3 times.
To separate the protein from the fat add 35 mL of acetone to the precipitate.

Add 10 mL of 0.5 M Ca(NO3)2 and heat on a low setting. Continue until volume has been reduced by roughly one third.
Allow to cool in an ice bath for about 5 minutes.
Filter the solid calcium phosphate using a Buchner Funnel. Wash with 2 mL of cold water then with 2 mL of ethanol and then 5 mL of acetone.
Dry the precipitate until it is easily removed from the filter paper and scrape onto another watch glass.