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Step 2: extracting the PGM enzyme contained within seeds of Brassica oleracea                 Obtain one seed of the cultivar assigned to your group Obtain a ceramic spot plate and label it with your name and name of cultivar place the labelled plate on top of ice remove dark seed coat place seed in one of the wells with 70 micro liters of grinding buffer       do not remove from ice until you are ready to transfer to sample plate! grind the tissue using a clean glass test tube until you have a yellow solution that does not have any intact tissue