Show a process.
|
|
|
|
|
|
|
|
|
|
|
|
Add about 50 mL of milk to a beaker. Record mass.
|
|
Add about 1 mL of 8 M Acetic Acid to the milk and stir while heating on hotplate at medium setting.
|
|
Once it boils remove and let cool for about 10 minutes.
|
|
Pour the beaker through a cheese cloth and into a 250 mL beaker. Squeeze the cheese cloth with the fat in it to get excess milk out.
|
|
Place precipitate from cheese cloth into a 150 mL beaker.
|
|
|
|
|
|
|
|
|
|
|
Add about 7mL of 1 M NaOH to the filtrate and test with litmus paper to ensure it is basic.
|
|
Place casein on a watch glass.
|
|
Separate the casein from the fat by using your buchner funnel. and wash with 5 mL of acetone.
|
|
Break the precipitate apart and then mix while on hot plate at low setting until it boils. take off and let cool for 2 minutes. Repeat 3 times.
|
|
To separate the protein from the fat add 35 mL of acetone to the precipitate.
|
|
|
|
|
|
|
|
|
|
|
Add 10 mL of 0.5 M Ca(NO3)2 and heat on a low setting. Continue until volume has been reduced by roughly one third.
|
|
Allow to cool in an ice bath for about 5 minutes.
|
|
Filter the solid calcium phosphate using a Buchner Funnel. Wash with 2 mL of cold water then with 2 mL of ethanol and then 5 mL of acetone.
|
|
Dry the precipitate until it is easily removed from the filter paper and scrape onto another watch glass.
|
| |
|
|
|
|
|
|
|
|
|
| |
| |
| |
| |
| |
|
|
|
|
|
|
|
|
|
| |
| |
| |
| |
| |
|
|
|
|
|
|
|
|
|
| |
| |
| |
| |
| |
|
|
|
|
|
|
|
|
|
| |
| |
| |
| |
| |
|
|
|
|
|
|
|
|
|
|
Milk Lab
