Before you decide
Over 20,000 selectors
asturgill's flowchart create and edit page

▷ ▶ Light driving Photosynthesis free Science & Nature flowcharts and decision trees.
Light driving Photosynthesis
A Flowchart by asturgill. See asturgill's 4me blog page.
Viewed 1885 times. Created September 2013.

Information and items from you may find helpful. This Science & Nature flowchart, a free online decision tool is a creation of asturgill and Science & Nature for amusement purposes only. The implicit and explicit opinions expressed here are the author's. does not necessarily agree.
Share your comments on your '4me' member page.
Be the first to comment.
Cookies Consent Information    Share
Search for more flowcharts Free: Make a flowchart
Will you share your opinion of this flowchart?

Set wavelength of spectrophotometer to 605 nm
put 15 ml of CMS into 30 ml cold beaker with a plastic beaker as an ice bucket on the bottom
Obtain one tube of DCIP and 15 ml of water in flask
obtain 7 test tubes. First one labelled B for blank and the red #1-6
Add 0.5 ml of water and 2.5 CMS to blank tube

place tube #1 in foam pad with the lights off
Cover with parafilm, mix well and record absorbance and time.
Tube #1- 0.5 ml DCIP and 2.5 ml of water
Use blank to zero spectrophotometer
Cover blank with parafilm and mix well

turn on light and expose test tube #! for one minute
Leaving the light on and covering the tube, record test tube #1's absorbance
Repeat last two steps until you have 5 absorbances
Test tube #2- add 0.5 ml DCIP, and 2.5ml of CMS
Repeat steps 11-12 with test tube #2 five times and record all absorbances

Mix tube well then remove foil jacket and cap
Cover test tube #3 with parafilm along with the cap and aluminum foil jacket.
Test tube #3- add 0.5 ml of DCIP, and 2.5 ml of CMS
prepare a foil cap for test tube #3
Wrap test tube #3 with aluminum foil

quickly measure the absorbance of the tube at 605 nm
Put the jacket and cap back on test tube #3 and place it in the light box and record the time
turn on the light and record the absorbance every 1 minute 5 times while reducing the amount of light each time by removing the cap and jacket
Cover the light box with purple cellophane and turn on the light
Test tube #4- add o.5 ml DCIP and 2.5 ml of CMS and mix well

For test tube #6 repeat steps 24-28 with red cellophane
For test tube #5 repeat steps 24-28 with green cellophane
Record the absorbance and repeat step 27 until 5 readings are made.
Place test tube #4 under in the light box with the purple cellophane for 1 minute
Take the absorbance of Test tube #4

For test tube #7 repeat steps 24-28 with another colored cellophane
open Microsoft Excel and enter time(x-axis) and absorbance at 605 nm (y-axis) on adjoining columns
highlight the date and select chart wizard
Click XY scatter chart type
Highlight the columns button