Pipet 4 ml of 80% acetone into a 13 X 100 mm test tube
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add 4-5 drops of the pigment extract already prepared
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Place rubber stopper on test tube and invert the tube. "Should have light green color"
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Make the blank by putting 4 ml of 80% acetone in test tube
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set spectrophotometer wavelength to 380 nm and zero it using blank.
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plot absorbance vs. wavelength on Excel.
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repeat at 10 nm intervals in peak absorbance regions.
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Repeat procedure at 40nm intervals until you reach 740
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Record absorbance of first tube made with extract and write it in notebook.
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